Fig. 3.

Comparison of increased levels of total desmin protein versus total levels of S-nitrosylated desmin. A, total desmin protein level from nonpregnant (NP) and pregnant (P) USM was separated by SDS-polyacrylamide gel electrophoresis, and a Western blot was performed using a desmin-specific antibody. Total desmin was normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the fold increase was calculated to be 1.792. B, total desmin S-nitrosylation was determined from three independent nitrosyl-DIGE experiments using DeCyder software, and the fold increase was calculated to be 5.733. A Student's t test was performed to analyze the difference in means between groups, and the result was shown to be statistically significant with p = 0.0005. MW, molecular weight.