TABLE 2.
S-Nitrosylated proteins identified in guinea pig USM that are involved in contraction/relaxation dynamics
A total of 34 S-nitrosylated proteins were identified that are known to play a role in either cytoskeletal rearrangement or the regulation of contraction/relaxation in smooth muscle tissues, 15 of these are novel targets of S-nitrosylation not previously identified and 20 are selectively S-nitrosylated during pregnancy.
| Protein | Biological Process | Previously Identified | NP | P | UniProt |
|---|---|---|---|---|---|
| Transgelina,b | Actin binding, contraction | N | N | Y | Q01995 (TAGL_Hu) |
| Destrina,b | Actin depolymerization | Y (PMID: 18670085) | N | Y | P60981 (DEST_Hu) |
| Tubulin β chaina,b | Cytoskeletal | Y (PMID: 16418269) | Y | Y | P07437 (TBB5_Hu) |
| α-Actinin-1a,b | Cytoskeletal | Y C332 (PMID: 20585580) | N | Y | P12814 (ACTN1_Hu) |
| Desmina,b | Cytoskeletal | Y C333 (PMID: 20585580) | Y | Y | P17661 (DESM_Hu) |
| Actin, cytoplasmic 1a | Cytoskeletal | Y (PMID: 20585580) | Y | Y | P60709 (ACTB_Hu) |
| Actin, α skeletal musclea | Cytoskeletal | Y (PMID: 10961840) | Y | Y | P68133 (ACTS_Hu) |
| Tubulin β-2B chaina | Cytoskeletal | N | N | Y | Q9BVA1 (TBB2B_Hu) |
| Vinculina | Cytoskeletal actin binding | Y (PMID: 17704302) | N | Y | P18206 (VINC_Hu) |
| Cofilin-1a | Cytoskeletal actin binding | N | Y | Y | P23528 (COF1_Hu) |
| Filamin-Ca | Cytoskeletal actin cross-linker | Y (PMID: 20585580) | N | Y | Q14315 (FLNC_Hu) |
| Annexin A2a,b | Cytoskeletal adapter | Y (PMID: 12199706) | N | Y | P07355 (ANXA2_Hu) |
| PDZ and LIM domain protein 1a | Cytoskeletal adapter (interacts with α-actinins 1, 2, and 4) | N | N | Y | O00151 (PDLI1_Hu) |
| Profilin-1a | Cytoskeletal remodeling | Y (PMID: 18283105) | N | Y | P07737 (PROF1_Hu) |
| Vimentina,b | Cytoskeletal remodeling | Y C328 (PMID: 20585580) | Y | Y | P08670 (VIME_Hu) |
| Ras-related protein R-Rasa | Cytoskeletal remodeling | N | N | Y | P10301 (RRAS_Hu) |
| Calpain small subunit 1a | Cytoskeletal remodeling and signal transduction | N | N | Y | P04632 (CPNS1_Hu) |
| Heat shock protein β-1a,b | Cytoskeletal remodeling and signal transduction | Y (PMID: 18670085) | Y | Y | P04792 (HSPB1_Hu) |
| Filamin-Aa | Cytoskeletal scaffold actin binding | Y C717 (PMID: 20585580) | Y | Y | P21333 (FLNA_Hu) |
| Phosphoglucomutase-like protein 5a | Cytoskeletal; interacts with dystrophin and utrophin | N | N | Y | Q15124 (PGM5_Hu) |
| Ankyrin-3a | Cytoskeletal-membrane linker | Y (PMID: 20585580) | Y | N | Q12955 (ANK3_Hu) |
| Talin-1a | Cytoskeletal-membrane connector | N | Y | Y | Q9Y490 (TLN1_Hu) |
| Kinesin family member 17a | Microtubule-based movement | N | N | Y | A2A3Q7 (A2A3Q7_Hu) |
| Transforming growth factor β-1-induced transcript 1 proteina | Molecular adapter at focal adhesion complexes | N | Y | Y | O43294 (TGFI1_Hu) |
| Protein S100-A10a | Protein binding, induces the dimerization of ANXA2/p36 | N | N | Y | P60903 (S10AA_Hu) |
| Gi subunit α-2a | Signal transduction | N | N | Y | P04899 (GNAI2_Hu) |
| Myosin regulatory light polypeptide 9a | Smooth muscle contraction | ? (PMID: 20585450) | N | Y | P24844 (MYL9_Hu) |
| Myosin-11a | Smooth muscle contraction | ? (PMID: 20585450) | N | Y | P35749 (MYH11_Hu) |
| Myosin light polypeptide 6a,b | Smooth muscle contraction | ? (PMID: 20585450) | Y | Y | P60660 (MYL6_Hu) |
| Myosin light chain kinase, smooth musclea | Smooth muscle contraction | N | N | Y | Q15746 (MYLK_Hu) |
| Tropomyosin β-chaina,b | Smooth muscle contraction is regulated by interaction with caldesmon | Y C170 (PMID: 19447776) | Y | Y | P07951 (TPM2_Hu) |
| Tropomyosin α-4 chaina | Smooth muscle contraction is regulated by interaction with caldesmon | Y C170 (PMID: 18992711) | N | Y | P67936 (TPM4_Hu) |
| Calponin-1a | Smooth muscle contraction | N | Y | N | P51911 (CNN1_Hu) |
NP, nonpregnant estrogen-primed guinea pig; P, pregnant near term 60- to 67-day timed pregnant; Y, yes; N, no; Hu, human; PMID, PubMed identification number.
a
Proteins were identified by LTQ-Orbitrap LC-MS/MS.
b
Proteins were identified by MALDI TOF/TOF.