TABLE 3.
Effect of benzil (100 μM) on IC50 values of phospho-NSAIDs
The cells were preincubated with benzil for 30 min, followed by incubation with phospho-NSAIDs for 24 h. The IC50 values were determined by the MTT assay.
| Phospho-NASID | IC50 |
CES1/Control | CES2/Control | ||
|---|---|---|---|---|---|
| HEK293-Control + benzil | HEK293-CES1 + benzil | HEK293-CES2 + benzil | |||
| μM | |||||
| Phospho-sulindac | 35 ± 2 | 29 ± 1 | 0.8 | ||
| Phospho-ibuprofen | 86 ± 6 | 82 ± 5 | 0.9 | ||
| Phospho-naproxen | 69 ± 7 | 75 ± 5 | 63 ± 5 | 1.1 | 0.9 |
| Phospho-indomethacin | 41 ± 2 | 41 ± 2 | 1.0 | ||
| Phospho-tyrosol-indomethacin | 50 ± 3 | 62 ± 5 | 109 ± 8 | 1.2 | 2.2 |
| Phospho-valproic acid | 21 ± 1 | 19 ± 1 | 0.9 | ||