Figure 3.

Generation of Sgce sKO mice. (A) Strategies to generate Sgce sKO mice. Sgce loxP neo mice were prepared as described previously (17). Neo cassette was removed by crossing with FLP mice. FLP was removed by backcrossing to C57BL/6 mice to produce Sgce loxP mice. Sgce loxP male mice were crossed with Rgs9-cre female mice to produce Sgce sKO mice. The primer sites to amplify the exon 4-deleted locus (Δ) were shown by the arrow pair under the map. (B) A representative image of multiplex PCR-based genotyping. Top band is PCR product from cre. Middle band is for Sgce loxP locus and the bottom band is the Sgce WT locus. Lanes 1, 5, 6: Sgce loxP heterozygous mice; lanes 2, 3: Sgce sKO mice; lane 4: WT mouse; lane 7: Rgs9-cre mouse. (C) Striatum-specific deletion of Sgce exon 4 in Sgce sKO mice was confirmed by PCR using DNA isolated from each brain region. Top bands were PCR products of dopamine transporter gene as an internal control. The deletion of Sgce exon 4 (Δ) was detected only in the striatum as predicted.