Figure 1.

H3K27m3 and OCT4 expression in undifferentiated hESCs. Shown are representative immunofluorescence images of the HSF-6 hESC line at passage 51, (A) DAPI, (B) OCT4, (C) H3K27me3 (arrow indicating focal dot in some nuclei), (D) Merger of (A) and (C). (E and G) H3K27me3, (F, H and J) OCT4, with arrows indicating bright (Br) or dim (Di) OCT4-positive nuclei, (I) DAPI. (K) OCT4-positive (n = 247, Br and Di) nuclei were counted and results are shown as percentage of bright or dim nuclei with and without H3K27me3 foci. (L and O) X chromosome paint, (M and P) H3K27me3, (N) Merger of (L) and (M) and (Q) Merger of (O) and (P).