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Cryopreservation increases incidence of class III in new hESC lines. Three-color FISH for XIST (pink), UTX (green) and ATRX (red) was performed on (A) female NHDF 17914 fibroblasts, (B) UCLA1 at passage (p) (p24), (C) UCLA3 at p25, (D) UCLA4 at p20, (E) UCLA5 at p31. (F) Interphase nuclei were analyzed from at least five randomly selected colonies in each hESC line, and the total sum of nuclei in each category are shown. Y-axis corresponds to total number of nuclei counted.
