Figure 4.

Telomerase catalytic activity is not affected by X-DC-associated dyskerin mutations. (A) Schematic and rationale of the immunoprecipitation and normalization procedure. Each TRAP activity profile was determined with a calculated input anti-dyskerin immunoprecipitate, normalized for TERT content, shown immediately above the TRAP products. (B) Representative immunoprecipitation-western-TRAP experiment. There were no observable differences between telomerase holoenzyme activities isolated from WT versus X-DC isoforms. TRAP activity profiles of a 5-fold dilution series are shown. Each IP-western-TRAP experiment was repeated at least three times with similar results. We also performed TRAP assays with the same immunoprecipitates in a 2.5-fold dilution series, and similar results were obtained.