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. Author manuscript; available in PMC: 2013 Jan 6.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Dec 8;417(1):570–575. doi: 10.1016/j.bbrc.2011.12.007

Figure 4. Effect of CDDO-Me on cellular phosphatases and protein phosphatase PP2A activity.

Figure 4

A. LNCaP and PC-3 cells were treated with CDDO-Me (0.3–5 µM) for 20 h and cell lysates were analyzed for PTEN, PP2A and PHLPP1 by immunoblotting. B. Effect on PP2A activity in vivo. PP2A was immunoprecipitated from LNCaP and PC-3 cells after treatment with CDDO-Me (0.3–5 µM) for 20 h. PP2A IP was incubated with p-Akt and dephosphorylation of p-Akt was analyzed by western blotting. Each experiment was repeated two times.