Fig 5.

Intergenic region between citC and citA. Putative binding sites of the regulators FNR, NarL, ArcA, and CRP as well as citC and citA promoter regions are based on the Prodoric Virtual Footprint software (35). The positions of the CitB binding sites are taken from Ingmer et al. (13). Conserved putative DcuR binding motifs (light blue rounded boxes), consisting of a tandem repeat of (T/A)(A/T)(T/C)(A/T)AA (1), are located upstream of citC and citA, respectively. Note that DcuR and CitB binding sites overlap. The 652-bp DNA fragment for the mobility shift assays shown in Fig. 1 covers the complete intergenic region shown in the figure and extends to the sequence at the 5′ and the 3′ ends shown here.