Table 4.
Influence of DcuS, DcuR, CitA, and CitB on the expression of citC-lacZ and dcuB-lacZa
| Strain | Activity (MU) |
|||
|---|---|---|---|---|
|
citC-lacZ |
dcuB-lacZ |
|||
| Fumarate | Citrate | Fumarate | Citrate | |
| IMW548/IMW237 (wild type) | 3 ± 1 | 320 ± 20 | 1,040 ± 125 | 586 ± 60 |
| IMW553/IMW260 (ΔDcuS−) | 2 ± 1 | 215 ± 24 | 23 ± 11 | 29 ± 1 |
| IMW554/IMW238 (ΔDcuR−) | 2 ± 2 | 95 ± 24 | 7 ± 1 | 5 ± 1 |
| IMW549/IMW280 (ΔCitA−) | 12 ± 2 | 5 ± 2 | 1,035 ± 75 | 434 ± 64 |
| IMW550/IMW239 (ΔCitB−) | 11 ± 3 | 8 ± 1 | 1,209 ± 181 | 628 ± 77 |
a
The expression of citC-lacZ or dcuB-lacZ was tested in strains deleted of dcuS, dcuR, citA, or citB in the absence and presence of an effector (fumarate or citrate). E. coli strains were grown under anaerobic conditions in eM9 medium with glycerol (50 mM) and dimethylsulfoxide (DMSO) (20 mM) plus sodium fumarate (20 mM) or sodium citrate (20 mM), as indicated. Activities in Miller units (MU) are the results from at least four independent measurements, and the standard deviations are given.