Fig 4.

Mapping of the subsets of the nucleotides present in the MRS of the B. abortus 2308 mntH promoter that are required for its interaction with Mur in an EMSA. The concentration of Mur added to the reaction mixtures is indicated above the lanes; the specific synthetic oligonucleotides (labeled native, 1, 2, 3, 4, and 5) used in the EMSA reactions are indicated below the lanes, and the nature of the nucleotide substitution in each synthetic oligonucleotide used in these reactions is shown at the bottom of the figure.