Fig 4.

Increased ADEP activity against M. tuberculosis using efflux pump inhibitors. M. tuberculosis was grown in the presence of two different concentrations of ADEP2 only (■) or in combination with reserpine (▴), verapamil (*), or both (×) at concentrations of 12 μg/ml and 40 μg/ml, respectively. (A) Growth in 10 μg/ml ADEP2. Aerobic growth in stirred culture tubes was monitored over 16 days. (B) Growth in 15 μg/ml ADEP2. Aerobic growth in stirred culture tubes was monitored over 16 days. (C) MICs against compound IDR-10011 were determined against M. tuberculosis in liquid medium (in 96-well plates) in the presence/absence of 12 μg/ml reserpine and/or 40 μg/ml verapamil. The experiment was performed in triplicate, and results are presented as average with standard deviations.