Table 2.
Primersa
| Primer | Oligonucleotide sequence (5′ → 3′) |
|---|---|
| a1 | ATTAGGATCCAT8AT8GAYAC8TAYGT8CC8GA |
| a2 | TTAAGGTACC8CC8GA8AT8CC8GC8CCRTC |
| hso36 | AGCAATGGCTAGCAATGAACAGTTTAGCCCATCCCAAC |
| hso38 | CTAATTTTCAAGTAACACGCCCAACGGTAGTGAGCAAGGGCGAGGAG |
| hso39 | AGCAATCTCGAGTTACTTGTACAGCTCGTCCATGC |
| hso50 | CTTGATAATCGTCTCAACTGACT |
| hso51 | AGCTTAAGATCTAGAGCTCGAGGATCAAGGTTGCTGAAAGCAGTAAGAA |
| hso52 | GGAAATATTCATTCTAATTGGTAAGCGCCCCTATCATTTAGCACCTCCCT |
| hso53 | AATGCTACGATTGCTTCACAAAAT |
| kana-f | ATCCTCGAGCTCTAGATCTTAAGCTT |
| kana-r | ACTCCGGATGCATATGCATGCT |
| khb113 | CTACAGGGAAGAGACCAAGG |
| khb114 | CAAGCTTAAGATCTAGAGCTCCTTGGATAATGCCAGATTAGG |
| khb115 | GAGCTCTAGATCTTAAGCTTG |
| khb116 | CTCCGGATGCATATGCATGC |
| khb117 | GCATGCATATGCATCCGGAGGCTGTGTGGTATAATATAGAGAAC |
| khb118 | GTCAAGTCAAAAATATTGTCCTG |
| khb121 | GTGGTAGTTTAGGTTTGTCTG |
| khb122 | CAAGCTTAAGATCTAGAGCTCTGATATTACCATTATTAAACCCC |
| khb123 | GAAAAGAAATTTATAAGAGGACTTAAGTAGGCTTCTCAACAAAAGGAG |
| khb124 | CTGCTATTGTCCCATAAAATTC |
| ss1 | CACCTGTATTGGGTCCCTTG |
| ss2 | AGCTTAAGATCTAGAGCTCGAGGATCTGGGCCCACCTGTATTATTC |
| ss3 | GGAAATATTCATTCTAATTGGTAAGCGCAAGTCGCTTTCGCACTTCTC |
| ss4 | TTTCTATCAGACGGCCTTGC |
a
All primers were designed in the course of this study. 8, inosine.