Table 4.
Values of Tlag, Trelease, and ΔTrelease of multiple individual spores of various B. subtilis strains germinating with l-valinea
| Strain | Preparation | Mean value (min) ± standard deviation of |
No of spores |
Observation period (min) | |||
|---|---|---|---|---|---|---|---|
| Tlag | Trelease | ΔTrelease | Examined (% germinated) | Counted | |||
| 533 | Prep 1 | 25.4 ± 14.6 | 27.9 ± 14.8 | 2.6 ± 1.0 | 335 (95) | 145 | 90 |
| Prep 2 | 21.9 ± 12.0 | 24.4 ± 12.2 | 2.5 ± 0.9 | 341 (97) | 217 | 90 | |
| 533b | Prep 1 | 14.3 ± 15.8 | 21.5 ± 16.5 | 7.2 ± 4.7 | 217 (94) | 70 | 120 |
| 2066 | Prep 1 | 4.9 ± 2.7 | 10.3 ± 3.4 | 5.4 ± 1.9 | 413 (99) | 126 | 90 |
| Prep 2 | 8.5 ± 5.1 | 13.8 ± 5.6 | 5.4 ± 1.7 | 238 (100) | 116 | 90 | |
| 2421 | Prep 1 | 2.6 ± 1.0 | 7.4 ± 2.3 | 4.8 ± 2.1 | 329 (100) | 135 | 60 |
| Prep 2 | 2.7 ± 0.9 | 8.3 ± 2.7 | 5.6 ± 2.2 | 249 (100) | 123 | 60 | |
| 2307 | Prep 1 | 8.6 ± 8.9 | 23.4 ± 11.6 | 14.9 ± 8.5 | 286 (99) | 115 | 90 |
| Prep 2 | 8.7 ± 6.4 | 22.4 ± 8.8 | 13.7 ± 5.7 | 337 (99) | 105 | 90 | |
| 2422 | Prep 1 | 4.7 ± 5.1 | 15.8 ± 6.4 | 11.1 ± 4.2 | 293 (99) | 84 | 90 |
| Prep 2 | 4.9 ± 5.3 | 18.9 ± 8.1 | 14.0 ± 6.3 | 204 (97) | 134 | 90 | |
| 111 | Prep 1 | 20.8 ± 15.5 | 46.6 ± 23.1 | 25.8 ± 12.8 | 306 (96) | 101 | 120 |
| Prep 2 | 19.8 ± 12.7 | 47.4 ± 16.4 | 27.6 ± 8.8 | 252 (99) | 94 | 120 | |
| 112 | Prep 1 | 26.9 ± 16.8 | 29.6 ± 17.3 | 2.7 ± 1.1 | 233 (96) | 138 | 90 |
| Prep 2 | 29.2 ± 17.1 | 32.4 ± 17.4 | 3.1 ± 1.4 | 447 (96) | 154 | 90 | |
| 113 | Prep 1 | 19.3 ± 16.4 | 40.8 ± 21.1 | 21.5 ± 12.4 | 290 (99) | 81 | 120 |
| Prep 2 | 26.8 ± 13.4 | 50.5 ± 18.4 | 23.7 ± 13.5 | 409 (98) | 110 | 120 | |
a
Heat-activated spores adhering to a microscope slide were germinated with l-valine, and the kinetic parameters of spore germination were determined as described in Materials and Methods and the legend to Fig. 2.
b
These spores were germinated with 25 mM EDTA also present.