Table 5.
Mean values and standard deviations of Tlag, Trelease, and ΔTrelease of multiple individual spores of various B. subtilis strains germinating with dodecylaminea
| Strain | Preparation | Mean value (min) ± standard deviation of |
No. of spores |
Observation time (min) | |||
|---|---|---|---|---|---|---|---|
| Tlag | Trelease | ΔTrelease | Examined (% germinated) | Counted | |||
| 533 | Prep 1 | 80.9 ± 43.0 | 83.3 ± 43.5 | 2.3 ± 1.4 | 288 (51) | 90 | 180 |
| Prep 2 | 68.1 ± 47.5 | 69.8 ± 47.7 | 1.7 ± 0.8 | 259 (60) | 98 | 180 | |
| 2066 | Prep 1 | 28.1 ± 24.4 | 33.7 ± 24.6 | 5.6 ± 4.5 | 500 (98) | 85 | 180 |
| Prep 2 | 58.9 ± 40.0 | 65.6 ± 41.2 | 6.7 ± 4.5 | 332 (87) | 96 | 180 | |
| 2421 | Prep 1 | 5.4 ± 4.6 | 8.7 ± 5.1 | 3.4 ± 1.5 | 312 (99) | 100 | 180 |
| Prep 2 | 15.6 ± 20.5 | 20.1 ± 21.3 | 4.5 ± 3.9 | 311 (94) | 100 | 180 | |
| 2307 | Prep 1 | 50.3 ± 41.8 | 63.0 ± 42.0 | 12.7 ± 7.7 | 254 (81) | 94 | 180 |
| Prep 2 | 58.7 ± 46.3 | 71.5 ± 50.6 | 12.8 ± 7.8 | 377 (79) | 71 | 180 | |
| 2422 | Prep 1 | 16.9 ± 11.2 | 24.3 ± 11.1 | 7.4 ± 2.3 | 282 (99) | 72 | 180 |
| Prep 2 | 25.1 ± 27.1 | 33.1 ± 28.6 | 8.1 ± 4.4 | 300 (99) | 105 | 180 | |
| 111 | Prep 1 | 78.4 ± 38.0 | 97.6 ± 39.1 | 19.2 ± 11.0 | 454 (20) | 69 | 180 |
| Prep 2 | 73.8 ± 44.6 | 90.7 ± 47.0 | 16.8 ± 7.7 | 345 (47) | 70 | 180 | |
| 112 | Prep 1 | 78.8 ± 36.4 | 82.4 ± 36.4 | 3.6 ± 2.0 | 574 (26) | 75 | 180 |
| Prep 2 | 74.1 ± 45.8 | 76.5 ± 46.6 | 2.4 ± 1.7 | 374 (41) | 93 | 180 | |
| 113 | Prep 1 | 61.3 ± 38.2 | 75.6 ± 38.4 | 14.3 ± 7.6 | 505 (29) | 61 | 180 |
| Prep 2 | 62.9 ± 49.1 | 74.7 ± 53.8 | 11.8 ± 8.2 | 373 (33) | 72 | 180 | |
a
Spores of various strains adhering to a microscope slide were germinated with dodecylamine, and the kinetic properties of spore germination were determined as described in Materials and Methods and in the legend to Fig. 2.