Table 1.
Genes interrupted in transposon mutants with biofilm maturation deficiency
| Rv no.a | Gene name | Description | Functional category | Tn insertion (bp)b |
|---|---|---|---|---|
| Rv0021c* | Conserved hypothetical protein (possible 2-nitropropane dioxygenase) | Conserved hypotheticals | 147 | |
| Rv0151c | pe1 | PE family protein | PE/PPE | 837 |
| Rv0199* | Probable conserved membrane protein | Cell wall and cell processes | 126 | |
| Rv0252* | nirB | Probable nitrite reductase [NAD(P)H] large subunit (Fad flavoprotein) | Intermediary metabolism and respiration | 46 |
| Rv0940c | Possible oxidoreductase | Intermediary metabolism and respiration | 45 | |
| Rv1268c | Hypothetical protein | Conserved hypotheticals | 157 | |
| Rv2946c* | pks1 | Probable polyketide synthase | Lipid metabolism | 2,343 |
| Rv3124 | Probable transcriptional regulatory protein | Regulatory proteins | 31 | |
| Rv3177 | Possible peroxidase (nonheme peroxidase) | Virulence, detoxification, adaptation | 783 | |
| Rv3267 | Conserved hypothetical protein (CpsA-related protein) | Conserved hypotheticals | 1,497 | |
| Rv3347c | ppe55 | PPE family protein | PE/PPE | 5,354 |
| Rv3883c* | mycP1 | Membrane-anchored mycosin (serine protease) | Intermediary metabolism and respiration | Not determined |
a
*, statistically significant attenuation in M. tuberculosis biofilm assay (P < 0.5, Student's paired t test).
b
Tn insertion sites are reported relative to the first base of the coding sequence for each ORF.