Table 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant genotype or phenotypea | Source or reference |
|---|---|---|
| Bacterial strains | ||
| Escherichia coli XL10 | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F′ proAB lacIqZΔM15 Tn10(Tetr) Amy Cmr] | Stratagene |
| Enterococcus faecalis | ||
| JH2-2 | Rifr Fusr | 23 |
| OG1RF | Rifr Fusr | 10 |
| OG1X | Smr | 22 |
| T9 | Tetr | 21 |
| Bacillus subtilis subsp. natto DSM 4451 | Smr | DSMZb |
| Plasmids | ||
| pIP501 | Tra Cmr MLSr | 12 |
| pMSP3535VA | pVA380-1 and ColE1 replicons nisRK PnisA Kmr | 6 |
| pJPrelGFP | Prel gfp | 31 |
| pQTEV | Pt4lacIq His7 Ampr | 35 |
| pJPrelGFP-oriT | pJPrelGFP oriTpIP501 | This work |
| pVA-gfp-oriT | pMSP3535VA gfp oriTpIP501 t0-termpQTEV | This work |
a
MLSr, macrolide-lincosamide-streptogramin B resistance; Hte, high transformation efficiency; Amy, amylase.
b
DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen (German Collection of Microorganisms and Cell Cultures).