Table 2.
Reactor | Gene copy no. per ml of reactor vol |
Ratio | |
---|---|---|---|
16S rRNA gene | hzsA gene | ||
A1 | 3.7 × 107 | 2.9 × 107 | 1.3:1 |
A2 | 1.3 × 109 | 1.0 × 109 | 1.2:1 |
A3 | 1.2 × 109 | 2.4 × 108 | 4.9:1 |
A4 | 4.0 × 109 | 1.4 × 109 | 2.9:1 |
A5 | 2.2 × 109 | 7.9 × 108 | 2.8:1 |
A6 | 8.6 × 109 | 3.1 × 109 | 2.8:1 |
A7 | 5.9 × 109 | 6.0 × 108 | 9.8:1 |
A8 | 1.7 × 109 | 3.4 × 108 | 5.2:1 |
qPCR was performed with primer sets Amx694F-Amx960R and hzsA_1597F-hzsA_1857R (this study), targeting the 16S rRNA and hzsA genes. 16S rRNA qPCR data were extracted from the work of Hu et al. (8).