Table 1.
Results of the multiplex PCR (validating and applying the method) for detection of the isolates with the GC1237 genotype
| Purpose and method | No. of isolates with the following genotype: |
Total no. of isolates tested by method | ||
|---|---|---|---|---|
| Beijing GC1237 | Beijing but not GC1237 | Not Beijing | ||
| Validation | ||||
| Spoligotyping | 9a | 9a | 49 | 58 |
| Multiplex PCR | 8 | 1 | 49 | 58 |
| RFLP | 8 | 1 | 9 | |
| Test use | ||||
| Spoligotyping | 66a | 66a | 50 | 116 |
| Multiplex PCR | 56 | 10 | 50 | 116 |
a
Because spoligotyping does not discriminate between the Beijing GC1237 genotype and Beijing but not GC1237 genotype, these data correspond to the same isolates.