Table 4.
Performance of FilmArray RP for detection of characterized adenovirus serotypes at high and low viral loadsa
| Adenovirus type (ATCC no.) | High viral load |
Low viral load |
||||
|---|---|---|---|---|---|---|
| PCR CT value | PCR DNA copies/ml (log10) | FilmArray RP result | PCR CT value | PCR DNA copies/ml (log10) | FilmArray RP result | |
| Group A | ||||||
| ATCC HAdv-12 (VR-863) | 26.05 | 3.41 × 107 (7.53) | Positive | 36.24 | 2.12 × 104 (4.33) | Positive |
| Group B | ||||||
| LRRI 3p | 26.16 | 1.75 × 107 (7.24) | Positive | 37.43 | 1.46 × 104 (4.16) | Positive |
| ATCC HAdv-7 (VR-7) | 25.70 | 4.24 × 107 (7.63) | Positive | 35.34 | 9.89 × 104 (5.00) | Positive |
| ATCC HAdv-11 (VR-12) | 26.47 | 2.61 × 107 (7.42) | Positive | 35.90 | 2.63 × 104 (4.42) | Positive |
| LRRI 14p | 27.20 | 1.65 × 107 (7.22) | Positive | 36.51 | 3.62 × 104 (4.56) | Positive |
| ATCC HAdv-21 (VR-256) | 23.77 | 5.47 × 107 (7.74) | Positive | 37.24 | 3.03 × 104 (4.48) | Positive |
| ATCC HAdv-35 (VR-718) | 28.30 | 6.02 × 106 (6.78) | Positive | 37.97 | 8.25 × 103 (3.92) | Negative |
| Group C | ||||||
| ATCC HAdv-1 (VR-1) | 27.87 | 5.98 × 106 (6.78) | Positive | 35.91 | 7.03 × 104 (4.85) | Positive |
| ATCC HAdv-2 (VR-846) | 25.88 | 1.51 × 107 (7.18) | Positive | 37.45 | 9.35 × 103 (3.97) | Negative |
| LRRI 2 | 27.16 | 9.29 × 106 (6.97) | Positive | 37.24 | 1.64 × 104 (4.22) | Negative |
| LRRI 5p | 27.92 | 1.05 × 107 (7.02) | Positive | 35.15 | 1.12 × 105 (5.05) | Positive |
| ATCC HAdv-6 (VR-6) | 26.84 | 2.07 × 107 (7.32) | Negative | |||
| Group D | ||||||
| LRRI 10 | 27.18 | 9.16 × 106 (6.96) | Positive | 36.50 | 3.64 × 104 (4.56) | Positive |
| ATCC HAdv-19 (VR-254) | 25.24 | 2.18 × 107 (7.34) | Positive | 34.91 | 4.97 × 104 (4.70) | Positive |
| LRRI 20p | 26.37 | 1.06 × 107 (7.03) | Positive | 37.14 | 2.45 × 104 (4.39) | Negative |
| ATCC HAdv-29 (VR-272) | 26.77 | 8.27 × 106 (6.92) | Positive | 36.27 | 1.04 × 104 (4.02) | Positive |
| ATCC HAdv-32 (VR-625) | 27.70 | 7.13 × 106 (6.85) | Positive | 36.30 | 1.04 × 104 (4.02) | Positive |
| ATCC HAdv-37 (VR-929) | 26.41 | 1.57 × 107 (7.20) | Positive | 36.70 | 1.60 × 104 (4.20) | Negative |
| Group E | ||||||
| ATCC HAdv-4 (VR-4) | 24.46 | 3.54 × 107 (7.55) | Positive | 35.03 | 4.55 × 104 (4.66) | Positive |
| Group F | ||||||
| ATCC HAdv-40 (VR-931) | 26.89 | 1.17 × 107 (7.07) | Positive | 36.61 | 3.39 × 104 (4.53) | Positive |
| ATCC HAdv-41 (VR-930) | 27.20 | 9.61 × 106 (6.98) | Negative | |||
a
Abbreviations: ATCC, American Type Culture Collection; LRRI, Lovelace Respiratory Research Institute. Real-time PCR CT values and viral loads represent means of multiple replicates. Repeat testing was performed on all discordant results.