Table 5.
Comparative detection of coinfections by FilmArray RP and real-time PCR assaysc
| Total no. of infections | Distinct coinfection combination detected by laboratory-developed real-time PCR assays |
Total no. of coinfections | No. of discrepant coinfectionsa | Discrepant analyte(s)a | |||
|---|---|---|---|---|---|---|---|
| Analyte 1 | Analyte 2 | Analyte 3 | Analyte 4 | ||||
| Adenovirus | MPV | 2 | 2 | Adenovirus (two) | |||
| Adenovirus | Rhinovirus | 1 | 1 | Adenovirus | |||
| Adenovirus | IV A H1 | 2 | 1 | Adenovirus | |||
| Adenovirus | IV A H3 | 1 | 1 | Adenovirus | |||
| Adenovirus | PIV type 3 | 1 | 1 | Adenovirus | |||
| Adenovirus | RSV A | 1 | 0 | ||||
| Adenovirus | RSV B | 1 | 1 | Adenovirus | |||
| MPV | Rhinovirus | 1 | 1 | MPV | |||
| MPV | IV A 2009 H1 | 1 | 0 | ||||
| MPV | PIV type 1 | 1 | 1 | MPV | |||
| MPV | PIV type 3 | 1 | 0 | ||||
| MPV | M. pneumoniae | 1 | 0 | ||||
| Rhinovirus | IV A H1 | 1 | 0 | ||||
| Rhinovirus | IV A H3 | 1 | 0 | ||||
| Rhinovirus | IV A 2009 H1 | 3 | 0 | ||||
| Rhinovirus | PIV type 2 | 1 | 1 | PIV type 2 | |||
| Rhinovirus | PIV type 3 | 1 | 1 | PIV type 3 | |||
| Rhinovirus | RSV B | 2 | 1 | RSV B | |||
| Rhinovirus | Bocavirus | 2 | 0 | ||||
| Rhinovirus | B. pertussis | 6 | 0 | ||||
| IV A H1 | RSV B | 2 | 0 | ||||
| IV A H3 | IV B | 1 | 1 | IV B | |||
| IV B | Bocavirus | 1 | 0 | ||||
| PIV type 1 | Bocavirus | 1 | 0 | ||||
| PIV type 3 | RSV A | 1 | 0 | ||||
| Bocavirus | M. pneumoniae | 1 | 0 | ||||
| Adenovirus | MPV | PIV type 3 | 1 | 0 | |||
| Adenovirus | MPV | RSV B | 1 | 0 | |||
| Rhinovirus | PIV type 2 | RSV B | 1 | 1 | PIV type 2 | ||
| Rhinovirus | PIV type 2 | Bocavirus | 1 | 0 | |||
| Rhinovirus | RSV A | Bocavirus | 1 | 0 | |||
| Adenovirus | Rhinovirus | PIV type 3 | Bocavirus | 1 | 1 | Adenovirus | |
| Dual | 38 | 13 | 13/76b | ||||
| Triple | 5 | 1 | 1/15b | ||||
| Quadruple | 1 | 1 | 1/4b | ||||
| Coinfections | 44 | 15 | 15/95 | ||||
a
A discrepant coinfection or discrepant analyte was defined as one that was detected by real-time PCR but not detected by the FilmArray Respiratory Panel.
b
The denominator represents the expected number of detections for specimens containing two (i.e., 38 × 2), three (i.e., 5 × 3), and four (i.e., 1 × 4) pathogens, respectively.
c
Abbreviations: MPV, metapneumovirus; IV, influenza virus; PIV, parainfluenza virus; RSV, respiratory syncytial virus.