Table 1.

Overview of data obtained from three runs of standard curves with serial dilutions of a plasmid clone containing the target for P. jirovecii DNA detection^a

Plasmid concn (copies/μl)	Quantification cycle
	Mean	Minimum	Maximum	SD	CV (%)
109	6.88	6.53	7.09	0.24	3.5
108	10.41	10.22	10.51	0.11	1.0
107	14.16	13.74	14.47	0.27	1.9
106	17.72	17.26	17.96	0.32	1.8
105	21.03	20.85	21.14	0.12	0.6
104	24.54	24.19	24.73	0.24	1.0
103	28.21	27.72	28.50	0.32	1.1
102	31.97	31.32	32.46	0.48	1.5
10	34.91	34.02	35.75	0.68	1.9
1	39.32	38.98	39.57	0.20	0.5

^aEach dilution was analyzed in duplicate. The coefficients of variation (CV) show good reproducibility.