Fig 3.

EC₅₀ values of 14 antiretrovirals for cell-free HIV and HIV-infected effector cells using firefly luciferase measurement on R5M cells. One hundred TCID₅₀ of either cell-free virus, resting PBMC, activated PBMC, or macrophages was added to R5M target cells and incubated for 7 days in the presence of a serial dilution of the respective compound. Firefly luciferase was used to assess target cell infection, and the EC₅₀ was calculated using nonlinear regression analysis on data from at least three to six independent experiments (GraphPad Prism). Nonentry inhibitors (A) and entry inhibitors (B) are shown (Table 1). The error bars depict the 95% confidence intervals, while the numbers and brackets represent the fold changes between cell-free virus and cell-associated virus (black) or between two types of cell-associated virus (gray). Significant differences were calculated using a Z test with Bonferroni correction; ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001.