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. 2012 Feb;56(2):830–837. doi: 10.1128/AAC.05438-11

Fig 5.

Fig 5

Examination of filibuvir or VX-222 binding based on DSF spectroscopy. (A) Derivatives of the thermal denaturation profiles of 1bΔ21 and M423T in the presence of filibuvir. The molar ratio of filibuvir to the proteins is 1.2. DMSO was used as a vehicle control and was present in the reaction mixture at a final concentration of 4%. Each curve was derived from the mean value of three independent samples. The samples within a set had Tm(app)s that varied less than 0.2°C. (B) Derivatives of the thermal denaturation profiles of 1bΔ21 and I482L in the presence of VX-222. (C) Summary of the effects of filibuvir and VX-222 on the Tm(app) of the 1bΔ21 and the three mutant proteins with substitutions in the thumb II pocket.