Fig 7.

Competition binding experiments. The NS5B protein was immobilized to the surface of the SPR chip as previously described. Analytes were tested in dual injection mode, and the y axis was normalized at the beginning of the second injection. (A) First injection (1) for 90 s with 160 nM filibuvir, followed by a second 90-s injection (2) containing 160 nM filibruvir plus 160 nM VX-222. (B) Response after injection (1) for 90 s with 160 nM filibuvir, followed by a second 90-s injection (2) containing 160 nM filibruvir plus 160 nM ANA-598.