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OFX-inhibited DNA supercoiling assay. Relaxed pBR322 (0.3 μg) was incubated with GyrA (50 ng) and GyrB (50 ng) in the presence of the indicated concentration of OFX. Quinolone-inhibited supercoiling activity assay was performed with combinations consisting of WT GyrB-WT GyrA (A), GyrA-Ala91Val (B), GyrA-Asp95Gly (C), and GyrA-Asp95Asn (D). R and SC, relaxed and supercoiled pBR322 DNA, respectively.
