Table 1.

Nucleotide sequences of primers used in PCR

Primer name	Primer sequence (nucleotide positions)a
k-45	5′-GGCATATGACTGATATCACGCTGCCACCAG-3′ (1–25)
k-46	5′-ATAACGCATCGCCGCGGGTGGGTCATTACC-3′ (361–390)
k-47	5′-CACCCGCGGCGATGCGTTATACCGAGGCTCGGCTTACTCC-3′ (371–410)
k-48	5′-GGCTCGAGTTAATGATGATGATGATGATGACCGACACCGCCGTCGG-3′ (2471–2490)
k-52	5′-GGCATATGGCTGCCCAGAGGAAG-3′ (1–18)
k-53	5′-CTAACTCACGTGCTTTACGTGCAGCTATTC-3′ (1259–1288)
k-54	5′-CGTAAAGCACGTGAGTTAGTGCGTCGAAAAAGTGCC-3′ (1270–1305)
k-55	5′-GGCTCGAGCTAATGATGATGATGATGATGGACATCCAGGAAACGAACATCC-3′ (2013–2037)
k-59	5′-GCACGGCGACGTGTCGATTTATG-3′ (261–283)
k-60	5′-CATAAATCGACACGTCGCCGTGC-3′ (261–283)
k-61	5′-CATCGATTTATGGCACGTTAGTGC-3′ (272–295)
k-62	5′-GCACTAACGTGCCATAAATCGATG-3′ (272–295)
k-65	5′-CATCGATTTATAACACGTTAGTGC-3′ (272–295)
k-66	5′-GCACTAACGTGTTATAAATCGATG-3′ (272–295)

^aSix-histidine tag codons are underlined, and mutated codons are shown in bold type.