Table 4.
MICs of antibiotics (μg/ml), biocides (μg/ml), and tea tree oil and terpinen-4-ol (%, vol/vol) for three Staphylococcus strains serially subcultured with terpinen-4-ol or tea tree oila
Agent |
S. aureus NCTC 6571 |
S. aureus ATCC 25923 |
S. epidermidis ATCC 12228 |
|||||||
---|---|---|---|---|---|---|---|---|---|---|
Passage no. | MIC |
Passage no. | MIC |
Passage no. | MIC |
|||||
Control | With 0.1% T4ol | Control | With 0.1% T4ol | With 0.1% TTO | Control | With 0.2% T4ol | ||||
AMX | 19 | 0.12 | 0.06 | 18 | 0.12 | 0.12 | 0.12 | 14 | 1 | 0.5 |
22 | 0.25 | 0.25 | 20 | 0.5 | 0.25 | 17 | 1 | 0.5 | ||
CIP | 17 | 0.25 | 0.06 | 16 | 0.12 | 0.12 | 0.25 | 14 | 0.25 | 0.25 |
19 | 0.12 | 0.06 | 18 | 0.25 | 0.06 | 0.12 | 17 | 0.5 | 0.25 | |
22 | 0.12 | 0.06 | 20 | 0.25 | 0.06 | |||||
GEN | 17 | 1 | 2 | 16 | 0.5 | 0.25 | 0.5 | 14 | 0.12 | 0.5 |
19 | 1 | 1 | 18 | 0.25 | 0.12 | 0.12 | 17 | 0.25 | 0.25 | |
22 | 1 | 2 | 20 | 0.25 | 0.25 | |||||
TET | 17 | 0.12 | 0.06 | 16 | 0.12 | 0.12 | 0.25 | 14 | 0.5 | 0.5 |
19 | 0.25 | <0.03 | 18 | 0.25 | 0.12 | 0.25 | 17 | 1 | 0.5 | |
22 | 0.12 | 0.06 | 20 | 0.25 | 0.12 | |||||
VAN | 17 | 1 | 1 | 16 | 2 | 2 | 2 | 14 | 4 | 4 |
19 | 1 | 0.5 | 18 | 1 | 1 | 2 | 17 | 4 | 4 | |
22 | 0.5 | 0.25 | 20 | 1 | 1 | |||||
Benzalkonium | 17 | 0.5 | 1 | 16 | 2 | 0.5 | 2 | 14 | 2 | 1 |
Chloride | 19 | 1 | 1 | 18 | 2 | 0.5 | 1 | 17 | 2 | 1 |
22 | 1 | 1 | 20 | 2 | 0.5 | |||||
Triclosan | 19 | 0.06 | 0.03 | 18 | 0.06 | 0.03 | 0.03 | 17 | 0.03 | 0.06 |
22 | 0.12 | 0.12 | 20 | 0.25 | 0.12 | |||||
Tea tree oil | 17 | 0.5 | 0.25 | 16 | 0.12 | 0.25 | 0.25 | 14 | 0.5 | 0.5 |
19 | 1 | 0.5 | 18 | 0.5 | 0.5 | 0.5 | 17 | 0.5 | 0.5 | |
22 | 0.25 | 0.25 | 20 | 0.5 | 0.5 | |||||
Terpinen-4-ol | 17 | 0.25 | 0.5 | 16 | 0.25 | 0.5 | 0.5 | 14 | 0.5 | 0.5 |
19 | 0.5 | 0.5 | 18 | 0.5 | 0.5 | 0.25 | 17 | 0.5 | 0.5 | |
22 | 0.12 | 0.25 | 20 | 0.12 | 0.25 |
Boldface type indicates a difference in MIC of 4-fold or more for passaged and nonpassaged strains.