Fig 2.

(A) Bioactivity assay assessing proteolytic stability of occidiofungin. Activity is reflective of the CFU count/ml. Occidiofungin (8 μg/ml) was exposed to trypsin (black), chymotrypsin (gray), and pepsin (white) for 30, 60, and 120 min. Control 1, control 2, and control 3 are the reaction buffer with protease, the reaction buffer alone, and the reaction buffer with protease and protease inhibitors, respectively. (B) Confirmation of protease activity using BSA as a target substrate. BSA was exposed to protease for 30, 60, and 120 min, followed by separation through a 12% SDS-PAGE gel. The first and last lanes are controls and contain BSA in reaction buffer without added protease for 0 and 120 min, respectively.