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RP-1 and AA-RP-1 exposure leads to increased membrane permeability and phosphatidylserine exposure. L. major promastigotes were cultured in medium with or without 100 μg of RP-1 or AA-RP-1/ml or 1 μg of staurosporine (STS)/ml for the various times indicated. Cells were then stained with annexin V-FITC and propidium iodide and analyzed by flow cytometry. The results are representative of at least three independent experiments.
