Fig 3.

The NRES-mediated increase in the ToxR level does not require ToxS. Strains N16961 (wild type), SAC119 (toxR::kan), and ARM755 (toxS::kan) were grown overnight in LB broth and then subcultured at a 1:100 dilution in T medium with or without 12.5 mM NRES mix. Cells were harvested at mid-log phase, and whole-cell preparations were resolved by SDS–10% PAGE and stained by Coomassie blue (A) or immunoblotted by using polyclonal anti-ToxR antisera (B).