Fig 3.

Latex beads covalently linked to CPS inhibit the phagocytic capacity of macrophages. J774 macrophages were pretreated with either unlinked control beads or CPS-linked beads (Beads-CPS) for 30 min. Then, fluorescent latex beads were added for an additional 30 min. (A) Confocal analysis showing significant less internalized fluorescent latex beads when macrophages were pretreated with CPS-linked beads. (B) FACS analysis of the quantity of internalized fluorescent latex beads. Macrophages incubated with CPS-linked beads only were used as controls and showed no fluorescence shift on FL2. Cells pretreated with unlinked control beads showed high levels of fluorescent bead internalization. A significant reduction in mean fluorescence intensity was observed when cells were pretreated with CPS-linked beads. Histograms are representative of three independent experiments.