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Filipin partially disrupts entry of nonencapsulated S. suis in macrophages. J774 macrophages were either pretreated or not with filipin for 15 min and then infected with WT S. suis or with its nonencapsulated mutant strain (CPS−) for 1 h. Cells were stained with rat anti-LAMP1 antibody to show bacterial internalization (red) and with rabbit anti-S. suis antibodies (green) to stain bacteria. Filipin partially blocked CPS− S. suis phagocytosis by macrophages.
