Fig 3.

Orientation of antisense RIVET clones and location of detected antisense transcripts. (A) RIVET antisense clone EF0226/0225/0224. (B) RIVET antisense clone EF0909. (C) RIVET antisense clone EF2397/2398. The genomic orientations of three antisense RIVET clones (black arrows) that predicted the presence of antisense transcripts are shown. The relative locations of primers used to subsequently detect and quantify the antisense transcripts by quantitative RT-PCR are indicated by heavy (reverse primers, used for reverse transcription and amplification) and light (forward primers, used for amplification) half-arrows. The nucleotide start site of each ORF, as annotated in Table S1 in the supplemental material, is indicated at its 5′ end. Although the graphic is not drawn to scale, the arrows representing adjacent genes within a locus are proportional to one another.