Table 1.
Enterococcus faecalis strains and oligonucleotides used in this study
| Strain or oligonucleotide | Description or sequence | Reference(s) |
|---|---|---|
| Strains | ||
| OG1RF | Wild-type strain | 21 |
| OG1RF ΔproB | Markerless in-frame deletion of locus EF0038 | This study |
| OG1RF ΔebrA | Markerless in-frame deletion of locus EF1809 | 4; K. S. Ballering and G. M. Dunny, unpublished data |
| OG1RF Δeep | Markerless in-frame deletion of locus EF2380; also called JRC106 | 29 |
| OG1RF Δeep(pMSP3535-eep) | Complementation plasmid; eep ORF with RBS cloned under expression of nisin-inducible promotera | This study |
| Oligonucleotides | ||
| NotI+0038F | 5′-CCCCGCGGCCGCCAAACTTACTCTAGCACGACGATC | This study |
| NcoI+0038R | 5′-CCCCCCATGGTTCAGAATGTTTTGTATTGTAGCGATT | This study |
| 0038-2stepF | 5′-AGAAACGAGGCACTTATGAGAAACAGTGACTGATTTAAAGCAACTAGGGC | This study |
| 0038-2stepR | 5′-GCCCTAGTTGCTTTAAATCAGTCACTGTTTCTCATAAGTGCCTCGTTTCT | This study |
| EF2380 XhoI F | 5′-CTAGTCTCGAGTTAAAAGAAAAAGCGTTGAATATCG | This study |
| EF2380 SpeI R RBS | 5′-CTAGTACTAGTGAATGAAGGAAGAAGGACATCTATG | This study |
| RIVET forward | 5′-AGCGTCGACTCTAGAGATCCAG | 4 |
| RIVET reverse | 5′-TACCCGTGCGTAACCAAAAAGTCG | 4 |
| EF0223F | 5′-TCACCGACAACAACTAAAGCT | This study |
| EF0223R | 5′-CATTTGGAATTAGAAGACCGC | This study |
| EF0909F | 5′-TAAGTTTTTCTTGGTTGGTATAAG | This study |
| EF0909R | 5′-ATCTTTTTCATGATTATCACTTTG | This study |
| EF2398F | 5′-GTTACCTTCGATGAAAGCGTC | This study |
| EF2398R | 5′-TCCTGATGAGATCGATGTTGT | This study |
a
See reference 8.