Table 1.
Mutants of N. meningitidis group B strain H44/76
| Strain | Genotypea | Phenotype |
|---|---|---|
| Wild type | Wild type | B:15:P1.7,16, ST-32, and fHbp ID 1b |
| ΔfHbp mutant | fHbp::Erm | fHbp not expressed |
| ΔfHbp ΔNspA mutant | fHbp::Erm; nspA::Spc | fHbp and NspA not expressed |
| Δlst mutant | lst::Kan | α-2,3-Sialyltransferase (lst) interrupted, prevents terminal LOS sialylation |
| ΔfHbp ΔNspA Δlst mutant | fHbp::Erm; nspA::Spc; lst::Kan | fHbp, NspA, and α-2,3-sialyltransferase not expressed |
a
Erm, erythromycin resistance; Spc, spectinomycin resistance; Kan, kanamycin resistance.
b
Capsular group, PorB serotype, and PorA variable region type, respectively. ST, multilocus sequence type. The fHbp identification numbers are the same as those designated on the website (http://pubmlst.org/neisseria/).