Fig 4.

MAbs 1E11 and 2B11 exhibit different neutralization mechanisms. (a) In consecutive incubation experiments, MAb 2B11 (light gray bars) is only effective when added together with NheB in the second incubation step (I) or during priming with NheB and NheC (II). MAb 1E11 (black bars) shows complete neutralization capacity in all experimental setups (I to IV). An unrelated MAb (5B2, dark gray bars) was used as a control. (b) Different combinations of components and the order of incubation steps used. Unbound Nhe components and antibodies were washed off after each incubation step. All data represent the means and standard deviations of six experiments.