Table 1.
Peptide fragments used in epitope-mapping experiments and reactivity of the MAbsa
| Fragment (aa start-end) | Primer (5′-3′ sequence) |
EIA titerb |
||
|---|---|---|---|---|
| Forward | Reverse | MAb 2B11 | MAb 1E11 | |
| N1 (31-372) | N1for (CACCAAAGATGCAATGGAAAGAAC) | Bflrev (TTATGCTTTTTTCGTATCTACTTTAATAT) | 640 | >640 |
| N2 (61-372) | N2for (CACCAATGTATCGTCTGTTGATG) | Bflrev (TTATGCTTTTTTCGTATCTACTTTAATAT) | 320 | >640 |
| N3 (93-372) | N3for (CACCCCACAGCTTATTTCAACG) | Bflrev (TTATGCTTTTTTCGTATCTACTTTAATAT) | 320 | >640 |
| N4 (122-372) | N4for (CACCGCAAAGGATAAAGCAACT) | Bflrev (TTATGCTTTTTTCGTATCTACTTTAATAT) | 160 | >640 |
| N5 (152-372) | N5for (CACCGACCTGAAGAAATTCCG) | Bflrev (TTATGCTTTTTTCGTATCTACTTTAATAT) | – | >640 |
| C1 (205-258) | C1for (CACCATTATTATCGGTTCATC) | C1rev (CTTGCGTAATACGATTCCAG) | – | – |
| C2 (251-324) | C2for (CACCACAGCTGGAATCGTATTA) | C2rev (AAGTAAAGAATTGTATTTTGATCC) | – | – |
| C3 (314-372) | C3for (CACCTACACAATGGGATCA) | C3rev (TGCTTTTTTCGTATCTACTAC) | – | >640 |
a
Amino acid (aa) numbering according to Granum et al. (14), without signal peptide. Gray shading in the forward primer sequences indicates additional nucleotides needed for directional cloning approach.
b
Reciprocal dilution. –, Negative at the lowest dilution tested (1:5).