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Both QseC and QseE regulate the LEE genes and nleA. qRT-PCR analyses of tir (LEE5) (A), eae (LEE5) (B), espA (LEE4) (C), and nleA (D) transcription. The mRNA levels for all of these genes were quantified and normalized to the mRNA levels of the endogenous internal control gene, rpoA. The mRNA levels were graphed as fold changes compared to wt transcript levels. The results are from at least three independent samples.
