Fig 8.

Motility regulation is QseC dependent but QseE independent. (A) Tryptone motility plates with the wt strain and the ΔqseC, ΔqseE, ΔqseC ΔqseE mutants and their complemented strains. (B) Representation of the diameter of the bacterial halos. (C) β-Galactosidase assays were performed using plasmid pVS177 with an fliC::lacZ promoter fusion in the wt and ΔqseC, ΔqseE, and ΔqseC ΔqseE mutant strains. (D) Representation indicating the QseC-dependent and QseE-independent activation of motility genes. QseC phosphorylates QseB, which directly binds to the regulatory region of flhDC, encoding the master regulators of flagella, leading to increase fliC expression, production of flagella, and motility (10).