Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Reference or source | Description |
|---|---|---|
| Strains | ||
| Wild type (wt) | 18 | wt O157:H7 (86-24 clinical isolate) |
| VS138 | 72 | ΔqseC |
| VS179 | 72 | ΔqseC strain complemented with pqseC (pVS155) |
| NR01 | 62 | ΔqseE |
| JN080 | This study | ΔqseE strain complemented with pqseE (pJN62) |
| JN07 | This study | ΔqseC ΔqseE |
| JN071 | This study | ΔqseC ΔqseE strain complemented with pqseC and pqseE |
| JN18 | This study | ΔrcsB |
| JN081 | This study | ΔrcsB strain complemented with prcsB (pJN63) |
| Plasmids | ||
| pKD4 | 11 | λ Red template plasmid |
| pKD46 | 11 | λ Red helper plasmid |
| pCP20 | 11 | λ Red helper plasmid |
| pBAD-myc-hisA | Invitrogen | C-terminal Myc-His tag cloning vector |
| pBAD33 | 20 | cloning vector |
| pRS551 | 68 | lacZ reporter gene fusion vector |
| pVS155 | 72 | qseC in pBADmyc His |
| pJN62 | This study | qseE in pBAD33 |
| pJN63 | This study | rcsB in pBADmyc His |
| pVS175 | 70 | fliC::lacZ in pRS551 |
| pVS182 | 70 | flhD::lacZ in pRS551 |