Table 2.
Oligonucleotides used in this study
| Oligonucleotide name | Description/use | Oligonucleotide sequence |
|---|---|---|
| yfhKP1 | qseE deletion for JN07 | GGCAAAGCCTGAATGCGCCTTAGCGACCAGGCGGCGCTGGTCAACCGCACCACGCTTATCGATGCCCGGCGCAGCGAAGCAATGACCAACGCGGCGCTGGATGTAGGCTGGAGCTGCTTC |
| yfhKP2 | TTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTATATGAATATCCTCCTTA | |
| JrcsB redF | rcsB deletion for JN18 | CGTGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGTGTAGGCTGGAGCTGCTTC |
| JrcsB redR | CGCCAGCTTCATCATCGCAGATTTCTTCTGGCTACTGATGGTTTTAATACTGCATATGAATATCCTCCTT | |
| JrcsB checkF | Deletion check for rcsB | CGAACCAGTGACTTTGCTGCGTTAGC |
| JrcsB checkR | CGCTGTTGAAATAATGGGAATCGTAGGACGGA | |
| JqseE bad33F | Complement plasmid pJN62 | GCTCTAGAGGCTATTCGCGTCTGACGAGAGTA |
| JqseE bad33R | CCCAAGCTTTTATTTCGTGTTTTTCGACGACGGTAATTCAATG | |
| JrcsB mycF | Complement plasmid pJN63 | CTCGGTACCAACAATATGAACGTAATTATTGCCGATGACCA |
| JrcsB mycR | CTCGAATTCGTCTTTATCTGCCGGACTTAACGTTACTG | |
| rpoA RTF | rpoA RT-PCR | GCGCTCATCTTCTTCCGAAT |
| rpoA RTR | CGCGGTCGTGGTTATGTG | |
| espA RTF | espA RT-PCR | TCAGAATCGCAGCCTGAAAA |
| espA RTR | CGAAGGATGAGGTGGTTAAGCT | |
| ler RTF | ler RT-PCR | CGACCAGGTCTGCCCTTCT |
| ler RTR | GCGCGGAACTCATCGAAA | |
| tir RTF | tir RT-PCR | CCATGGAGAGCAGACGTAGCT |
| tir RTR | CGGTGATCCTGGATTTAACCTT | |
| eae RTF | eae RT-PCR | GCTGGCCTTGGTTTGATCA |
| eae RTR | GCGGAGATGACTTCAGCACTT | |
| nleArt549F | nleA RT-PCR | AGCCACTACTTCGACGGTAACC |
| nleArt624R | ACGAACCACTTGAGCTGTTAATCC | |
| rcsBF | rcsB RT-PCR | TCTCTCGCCAAAAGAGAGTGAAG |
| rcsBR | CGATCTCGGTCACCAGGAA | |
| qseC RT1 443F | qseC RT-PCR | AATGGGAATACCGTGAAGACATG |
| qseC RT1 505R | CCAACCACGGGATCAATTG | |
| QseE RTF | qseE RT-PCR | CCCTTCACCGCCCCTTT |
| QseE RTR | CGCGCCATGATCTTCGA |