Fig 4.

Coimmunoprecipitation of HVEM(200t) and nectin-1(346t) by anti-gD MAbs. Reaction mixtures of gD2(285t) in the presence (+) or absence (−) of purified truncated nectin-1(346t) (A) or HVEM(200t) (B) were immunoprecipitated by the indicated MAbs. DL6 and DL11 were used as positive and negative controls, respectively. For nectin-1, the Western blots were probed with anti-gD and anti-nectin-1 polyclonal Abs. For HVEM, two gels, one for HVEM and the other for gD, were processed after the coimmunoprecipitation, and each blot was probed. Secondary Abs were then added, and enhanced chemiluminescence was used to visualize the bands.