Fig 5.

Effect of MAbs on binding of gD2(306t) to HVEM and nectin-1. Serial 2-fold dilutions of each MAb IgG were incubated for 1 h with a constant amount of gD2(306t). The gD-IgG mixture was then added to an ELISA plate on which either HVEM (A and C) or nectin-1 (B and D) had previously been immobilized. Binding of gD was then detected with polyclonal antibody R8 followed by secondary antibody and substrate. The results are presented in separate panels for clarity (note the differences in the y-axis scale values). Results were normalized to the amount of gD bound following incubation with a control IgG (anti-myc). Results of a representative experiment are shown; each antibody was tested a minimum of three times.