Fig 6.

Effect of MAbs MC2 and MC5 on receptor binding to gD2(306t) or gD2(285t). Serial 2-fold dilutions of the two forms of gD2 were mixed with antibody MC2 (A and B), MC5 (C and D), or MC14 (E and F) at a concentration of 0.5 μM for 1 h. The gD-IgG mixture was then incubated with purified receptor that had been immobilized to an ELISA plate. Binding of gD was detected with polyclonal anti-gD antibody R8 and anti-rabbit secondary antibody. Results of a representative experiment are shown; each antibody was tested a minimum of three times.