Fig 3.

One-step growth curves of the Ac-Δvp80 genome repaired with different VP80 truncation variants. Sf9 cells were transfected with 5.0 μg of DNA of either N-terminally truncated (Ac-Δvp80-vp80ΔN1, Ac-Δvp80-vp80ΔN2, Ac-Δvp80-vp80ΔN3, and Ac-Δvp80-vp80ΔN4) (A) or C-terminally truncated (Ac-Δvp80-vp80ΔC) (B) repaired bacmids. For all of these constructs the viral propagation was compared to that of Ac-Δvp80-vp80 coding for full-length VP80 protein. Cell culture supernatants were harvested at the indicated times p.t. and analyzed for release of infectious BV by endpoint dilution. Infectivity was determined by monitoring EGFP expression. The points indicate the averages of titers derived from three independent transfections, and the error bars represent the standard deviations (SD).