Fig 3.
Growth rates and viral protein expression of rMuVIowa/US/06ΔV, rMuVIowa/US/06, and MuVIowa/US/06. (A) Growth rates of rMuVIowa/US/06ΔV and rMuVIowa/US/06 in Vero cells. Vero cells were mock infected or infected with rMuVIowa/US/06ΔV or rMuVIowa/US/06 at an MOI of 0.01. Supernatants were collected for plaque assay. (B) Plaques of rMuVIowa/US/06ΔV and rMuVIowa/US/06 in Vero cells. rMuVIowa/US/06ΔV or rMuVIowa/US/06 was plated onto Vero cells. The plaques were stained with Giemsa at 6 dpi. (C) Viral protein expression of rMuVIowa/US/06ΔV and rMuVIowa/US/06 in Vero cells. Vero cells were infected as in panel A. Viral protein levels were examined by immunoblotting with anti-NP and P. β-Actin was used as a loading control. (D) Growth rates of rMuVIowa/US/06ΔV and rMuVIowa/US/06 in HeLa cells. HeLa cells were infected as in panel A. (E) Growth rates of rMuVIowa/US/06ΔV and rMuVIowa/US/06 in 293T cells. 293T cells were mock infected or infected with rMuVIowa/US/06ΔV or rMuVIowa/US/06 at an MOI of 0.5. Supernatants were collected for plaque assay.