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. 2012 Feb;86(3):1768–1776. doi: 10.1128/JVI.06019-11

Fig 4.

Fig 4

Ratios of NP and P in rMuVIowa/US/06ΔV-infected cells. (A) NP and P expression levels in rMuVIowa/US/06ΔV- and rMuVIowa/US/06-infected Vero cells during early time points postinfection. Vero cells were mock infected or infected with rMuVIowa/US/06ΔV or rMuVIowa/US/06 at an MOI of 0.5. Vero cells were collected and examined for NP and P expression using flow cytometry. Ratios of mean fluorescence intensity (MFI) of P over NP are shown. P values of rMuVIowa/US/06 versus rMuVIowa/US/06ΔV at 12 and 16 hpi were calculated using Student's t test and are less than 0.05. (B) NP and P expression levels in rMuVIowa/US/06ΔV- and rMuVIowa/US/06-infected Vero cells during late time points postinfection. Ratios of MFI of P over NP at multiple time points postinfection were examined as in panel A. P values of rMuVIowa/US/06 versus rMuVIowa/US/06ΔV at 24 and 48 hpi are less than 0.05. (C) NP and P expression ratios of rMuVIowa/US/06ΔV (P GS). Ratios of MFI of P over NP in rMuVIowa/US/06ΔV (P GS)-infected Vero cells, at an MOI of 0.5, were examined at 24 hpi. P values of rMuVIowa/US/06 versus rMuVIowa/US/06ΔV (P GS) are less than 0.05. (D) NP and P expression ratio of rMuVIowa/US/06ΔV (L gene). Ratios of MFI of P over NP in rMuVIowa/US/06ΔV (L gene)-infected Vero cells, at an MOI of 0.5, were examined at 24 hpi. P values of rMuVIowa/US/06 versus rMuVIowa/US/06ΔV (L gene) are less than 0.05.