Fig 2.

Interaction between vIL-6 and VKORC1v2. Association of vIL-6 with VKORC1v2 was identified by coprecipitation of vIL-6 with chitin bead-precipitated VKORC1v2-CBD from lysates of transfected HEK293T cells. Precipitated proteins (Ppt) were identified by immunoblotting (IB) for detection of vIL-6 and CBD. The two bands for vIL-6 represent glycosylated and unglycosylated forms of the protein. VKORC1v1-CBD did not precipitate vIL-6 (left). The right panels present the results of an analogous experiment, showing specificity of VKORC1v2 binding to vIL-6; no interaction between VKORC1v2 and ER protein calreticulin (Crt) was detected. vec, empty vector.