Fig 7.

Role of the VKORC1–vIL-6 interaction in PEL cell growth and survival. (A) BCBL-1 cells were coinfected with lentivirus vectors specifying control (NS) or VKORC1v2-specific shRNA and “shRNA-resistant” (sh^r) wild-type or DY₃₉AA-mutated (vIL-6-refractory) VKORC1v2 proteins, or wild-type VKORC1v2 susceptible to shRNA targeting (control for effective depletion). Cell growth was monitored daily following lentivirus transduction and a 2-day rest by counting of trypan blue-excluding cells. (B) In a parallel experiment, apoptosis was assayed at day 4 based on Annexin V-Cy3 staining and calculated as the fraction of total cells in each of >10 random fields. For growth and apoptosis data, error bars represent deviations from mean values obtained from triplicate cultures; t test values (unpaired, two tailed) are shown in panel B.